Fractionation of Yellow Thatching Grass (Hyparrhenia filipendula) for Sugar Production Using Combined Alkaline and Deep Eutectic Solvent Pretreatment

Biomass pretreatment followed by enzymatic hydrolysis is one of the most viable ways to obtain sugars from biomass. In this work, effect combined alkaline and Deep Eutectic Solvent on enzyme Hyparrhenia filipendula cellulase investigated. There no previously reported literature substrate authors aim establish baseline values for further research in utilization filipendula. The yellow thatching grass (Hyparrhenia filipendula) was fractionated with a combination increase sugar recovery. An solution 10 wt % (w/v) Sodium Hydroxide (NaOH) used first stage at 100 °C 4 h. Three DES, namely, Choline chloride (ChCl): urea; ChCl: glycerol; Ethylene glycol: Citric acid 1:2 molar ratio each, were heated 80 until clear formed. DESs second 110 6 h Parr reactor. During pretreatment, solid: solvent 1:10 stages pre-treatment. Enzymatic accomplished blend, Cellic CTec2, 50 mM sodium citrate buffer (pH 4.8) using shaking incubator speed 150 rpm. A solid loading 2% dosage g/100 g cellulose sample all experiments. Furthermore, samples withdrawn every 24 7 days analyzed glucose xylose High-Performance Liquid Chromatography (HPLC). high delignification 90% hemicellulose removal 70% achieved Alkali Urea pretreatment. Subsequently, highest conversion 92% observed, respectively, same sample. Additionally, yield 25 gL−1 alkaline/ChCl: Glycerol treated after 120 Moreover, 3 raw sample, NaOH- pulped Glycerol-treated results study demonstrated that solvents fractionating biomass have significant recovery during hydrolysis. Also, NaOH DES effective total sugar. conclusion, promising bio-refineries. However, ideal conditions should be investigated promote its value-added products.